五种微生物发酵剂对玉米芯的发酵效果

为了筛选出适合玉米芯发酵的微生物菌剂,以不加菌剂为对照,研究了添加5种微生物菌剂对玉米芯的发酵腐熟效果和发酵过程中堆料温度、体积、营养的动态变化。结果表明:添加外源微生物菌剂能有效加快玉米芯发酵并显著影响营养元素的动态变化,其中添加"金宝贝"菌剂促进发酵的效果最好。5种处理中"金宝贝"升温速度最快,最高温度达52.6℃,50℃高温可持续5 d以上,且体积下降百分比最高达12%之多,速效营养释放量最高。与对照相比,各处理发酵至30 d时异味消失体积下降明显且表层出现大量菌丝,达到腐熟。5种处理全氮与碱解氮含量变化正好相反:全氮含量先下降后上升而速效氮含量先增加后减少;全磷、速效磷、全钾、速效钾含量均呈上升趋势;而对照升温缓慢、营养元素含量变化不明显。     

沼液在水稻秧苗培育中的应用

研究了不同浓度沼液对杂交水稻种子浸种和秧苗培育的影响,结果表明,使用沼液比重在1.002-1.005g/cm3,对杂交稻种子发芽率、成秧率和秧苗生长质量都有良好的促进作用,同清水对照相比,其发芽率、成秧率和秧苗生长质量各项指标都达到显著或极显著,但使用超过比重为1.009g/cm3时都有明显的抑制作用,发芽率降低,秧苗生长不均匀。     

水泥刨花板研究进展

阐述了不同植物材料与水泥的水化特性、改善相容性以及水泥刨花板生产工艺的国内外研究现状,并对水泥刨花板的发展在适应原料的多样化、技术设备的升级及在建筑材料领域的应用等进行展望。     

融水县2004年第3代三化螟大发生原因分析

三化螟[Tryporyza incertulas（walker）]是融水县水稻生产上的一种历史性主要害虫之一，它的发生与为害程度大小对水稻产量影响极大。近40a来第3代的发生，除1975、1986、1999年为大发生之外，其它年份均属中等程度发生。2004年第3代继上述3a大发生之后再度暴发，发生面积达9333．3hm^2，比1986、1999年同代发生减少2933．3hm^2，3333．3hm^2，     

芜菁花叶病毒不同分离物3'-cDNA片段的克隆及序列分析

 从河北、北京、山东泰安和潍坊等地区的萝卜、大白菜、甘蓝、油菜上得到8个芜菁花叶病毒(Turnip mosaic virus,TuMV)分离物,测定了它们3'-末端cDNA片段的序列。根据衣壳蛋白基因(cp)核苷酸序列可以将这8个分离物与另外2个TuMV分离物分为3组:泰安旧镇大白菜(JZBC)、甘蓝(JZGL)和萝卜(JZLB)分离物为一组;北京(BJILB)、潍坊萝卜分离物(WFLB)与引起萝卜红心病的TuMV分离物(WFLB99)为一组;泰安范镇、河北、潍坊(WFLB04)萝卜和泰安旧镇油菜(JZYC)上的TuMV分离物为一组。农壳蛋白氨基酸序列比较结果与此基本一致,所不同的是分离物WFLB99与所有分离物的差异均较大,形成单独一个分支。有必要对TuMV的变异情况和致病机理进行深入研究。     

猪传染性胃肠炎病毒分子生物学研究进展

猪传染性胃肠炎病毒(TGEV)基因组为不分段的单股正链RNA,其编码4种结构蛋白和3种非结构蛋白。TGEV S蛋白是基因工程研究的重点,近年来,S蛋白在大肠埃希菌、沙门菌、腺病毒等中得到了高效表达。将传染性胃肠炎病毒的基因组人工改造成为感染性cDNA的表达载体,此载体可在ORF3处插入外源基因,异源基因绿荧光蛋白可稳定、高效地表达。TGEV基因1和其他冠状病毒相比保守性很强,所以对TGEV聚合酶的研究,特别是其中保守酶的研究,对于抗TGEV及其他冠状病毒药物的研究有着重要的意义。     

胰腺干细胞的研究进展

概述了胰腺干细胞的分布、胰腺的发生、胰腺干细胞的相关标记物以及胰腺干细胞的体外分离培养、增殖、分化等领域的研究进展，并对胰腺干细胞的生物学特征、体外分离培养时遇到的困难等进行了探讨，针对所遇到的困难提出了一些改进意见。     

Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs

In order to identify the molecular markers related to alive litter size of Bama Xiang pigs,the genome-wide association study (GWAS) was used to map and screen the candidate genes affecting the alive litter size trait.Ear tissue samples of 297 Bama Xiang pigs with multiple parity records were collected,and DNA was extracted and genotyped by porcine 50K SNP beadchip.After quality control and genotype imputation,the alive litter size of Bama Xiang pigs were GWAS by Tassel.The results showed that the average number born alive per litter of Bama Xiang pigs increased gradually with the increasing of parity in the range of 1-9 parities.A total of 32 816 SNPs were obtained after quality control and filtration.8 SNPs related to alive litter size of Bama Xiang pigs were screened by genome-wide association analysis,which were significant at genome or chromosome level.Based on the enrichment analysis of the coding genes in the region between 500 kb upstream and downstream of the associated significant SNP loci,and the QTL regions and gene functions related to porcine reproductive traits,4 genes (CAPZB,MSH3,CITED2 and HSD17B7) were finally identified to be candidate genes related to alive litter size of Bama Xiang pigs.     

饲料水分的快速测定

叙述了饲料水分快速测定方法，用此法与国标法对饲料水分进行测定比较，筛选出以第2组烘干30min（135±2℃）为最佳组合，其测定结果准确，节省时间，重现性好。     

Expression of telomerase inhibitor Pinx1 in leukemia cells and its correlation with telomerase activity

AIM:To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells,and to realize its effect on telomerase activity.METHODS:Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA.The correlations between Pinx1 and hTERT expression were also analyzed.RESULTS:Pinx1 mRNA expression in acute leukemia samples (0.00312,5.42×10-4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89×10-4,0-0.00863,P<0.01).The expression of Pinx1 mRNA had significant positive correlation with hTERT mRNA expression (r=0.296,P<0.05).Pinx1 mRNA expression decreased during differentiation,its expression was positive correlated with hTERT mRNA expression (r=0.900,P<0.05).CONCLUSIONS:As an inhibitor of telomerase,however,Pinx1 also had the same direction of regulation with telomerase activity in acute leukemia cells,suggesting its expression variation may be a subsequent reaction induced by that of hTERT to stabilize telomerase activity.The exact mechanisms remained to be verified.